Mesenchymal stromal cells (MSCs), which can be derived from many different tissue sources including bone marrow and fat, have the potential for the treatment of a broad range of clinical conditions, ,,. Stimulated BMSCs grown in MesenCult-XF produced the most extensive network of capillary-like tube structures in an in vitro angiogenesis assay. Stimulated ASCs grown in MesenCult-XF evoked the longest neurite outgrowth in a neuron co-culture model. In vitro stimulation of the cells enhanced the expression of brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF-A) and angiopoietin-1. Expression levels of CD73 and CD90 were similar in both cell types under the various culture conditions but CD105 was significantly reduced at passage 10 in MesenCult-XF. BMSCs showed morphological changes at late passage in MesenCult-XF and stained positive for senescence β-galactosidase activity.

ASCs cultured in MesenCult-XF continued to expand at faster rates than cells grown in serum. However, CFUs were significantly lower in MesenCult-XF.

At early passage, both ASCs and BMSCs showed better proliferation in MesenCult-XF compared with standard α-MEM–containing serum.

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